Dynamics and control of DNA sequence amplification
نویسندگان
چکیده
منابع مشابه
Dynamics and control of DNA sequence amplification.
DNA amplification is the process of replication of a specified DNA sequence in vitro through time-dependent manipulation of its external environment. A theoretical framework for determination of the optimal dynamic operating conditions of DNA amplification reactions, for any specified amplification objective, is presented based on first-principles biophysical modeling and control theory. Amplif...
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In “Dynamics and Control of DNA Sequence Amplification”, we have formulated the PCR optimal control problem and discussed the strategies for solving it. In this chapter, we implement those strategies and solve a simple PCR optimal control problem. The foundational concepts of Optimal Control were developed as early as the 1750s in classical mechanics as the principle of least action, a variatio...
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A theoretical framework for prediction of the dynamic evolution of chemical species in DNA amplification reactions, for any specified sequence and operating conditions, is reported. Using the polymerase chain reaction (PCR) as an example, we developed a sequence- and temperature-dependent kinetic model for DNA amplification using first-principles biophysical modeling of DNA hybridization and po...
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ژورنال
عنوان ژورنال: The Journal of Chemical Physics
سال: 2014
ISSN: 0021-9606,1089-7690
DOI: 10.1063/1.4899053